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hcas9 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc hcas9 plasmid
    Hcas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 679 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcas9/hCas9+(Plasmid+%2341815)/us12595478-4604-10-12
    Average 96 stars, based on 679 article reviews
    hcas9 plasmid - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Corilagin induces pyroptosis in AML cells by activating the TXNIP-caspase-3-GSDME pathway
    Article Snippet: .. After 24 h, the cells were transfected with plasmids containing GSDME/caspase-3 (CASP3) sgRNA and hCas9 in combination with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) plasmids at a mass ratio of 4:3:2. ..

    Article Title: Corilagin induces pyroptosis in AML cells by activating the TXNIP-caspase-3-GSDME pathway.
    Article Snippet: .. After 24 h, the cells were transfected with plasmids containing GSDME/ caspase-3 (CASP3) sgRNA and hCas9 in combination with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) plasmids at a mass ratio of 4:3:2. ..

    Plasmid Preparation:

    Article Title: Signaling pathways contributing to the transcriptional response to alkalinization in Komagataella phaffii: role of the transcription factors Crz1 and Rim101.
    Article Snippet: .. This vector contains the S. cerevisiae CEN6/ARS4 sequence to allow episomal maintenance in K. phaffii, expresses hCas9 from the LAT1 promoter, and can be selected in the presence of hygromycin (Addgene # 104907). ..

    Article Title: Komagataella phaffii encodes two functional Pho4 transcription factors
    Article Snippet: .. This vector expresses hCas9 from the LAT1 promoter and can be selected in the presence of hygromycin (Addgene # 104907). ..

    Article Title: Signaling pathways contributing to the transcriptional response to alkalinization in Komagataella phaffii: role of the transcription factors Crz1 and Rim101
    Article Snippet: .. This vector contains the S. cerevisiae CEN6 / ARS4 sequence to allow episomal maintenance in K. phaffii , expresses hCas9 from the LAT1 promoter, and can be selected in the presence of hygromycin (Addgene # 104907). ..

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap.
    Article Snippet: .. Plasmid vectors for genome editing, which were used to measure electrical potential, were modified from pSB11Cas11-ELP1A5. hCas9 between the XbaI and AscI sites was replaced with zCas9i, which was amplified from pAGM55273 (Addgene, Plasmid #153211), with the XbaI site replaced by the SmaI site. .. An expression cassette composed of the 35S promoter, CAT1 intron, EGFP, and NOS terminator between the AgeI and PmeI sites was replaced with a cassette composed of the CmYLCV promoter43,44, GCaMP6f26 (Addgene, Plasmid #40755), and the Ps rbcS 3 A terminator45.

    Sequencing:

    Article Title: Signaling pathways contributing to the transcriptional response to alkalinization in Komagataella phaffii: role of the transcription factors Crz1 and Rim101.
    Article Snippet: .. This vector contains the S. cerevisiae CEN6/ARS4 sequence to allow episomal maintenance in K. phaffii, expresses hCas9 from the LAT1 promoter, and can be selected in the presence of hygromycin (Addgene # 104907). ..

    Article Title: Signaling pathways contributing to the transcriptional response to alkalinization in Komagataella phaffii: role of the transcription factors Crz1 and Rim101
    Article Snippet: .. This vector contains the S. cerevisiae CEN6 / ARS4 sequence to allow episomal maintenance in K. phaffii , expresses hCas9 from the LAT1 promoter, and can be selected in the presence of hygromycin (Addgene # 104907). ..

    other:

    Article Title: Treatment of Huntington’s disease with a pan-HTT-targeting CRISPR nuclease
    Article Snippet: SpCas9, SpCas9-NG and eSpCas9(1.1) were expressed from hCas9 (Addgene #41815), pX330-SpCas9-NG (Addgene #117919) and eSpCas9(1.1) (Addgene #71814), respectively.

    Modification:

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap.
    Article Snippet: .. Plasmid vectors for genome editing, which were used to measure electrical potential, were modified from pSB11Cas11-ELP1A5. hCas9 between the XbaI and AscI sites was replaced with zCas9i, which was amplified from pAGM55273 (Addgene, Plasmid #153211), with the XbaI site replaced by the SmaI site. .. An expression cassette composed of the 35S promoter, CAT1 intron, EGFP, and NOS terminator between the AgeI and PmeI sites was replaced with a cassette composed of the CmYLCV promoter43,44, GCaMP6f26 (Addgene, Plasmid #40755), and the Ps rbcS 3 A terminator45.

    Amplification:

    Article Title: MSL10 is a high-sensitivity mechanosensor in the tactile sense of the Venus flytrap.
    Article Snippet: .. Plasmid vectors for genome editing, which were used to measure electrical potential, were modified from pSB11Cas11-ELP1A5. hCas9 between the XbaI and AscI sites was replaced with zCas9i, which was amplified from pAGM55273 (Addgene, Plasmid #153211), with the XbaI site replaced by the SmaI site. .. An expression cassette composed of the 35S promoter, CAT1 intron, EGFP, and NOS terminator between the AgeI and PmeI sites was replaced with a cassette composed of the CmYLCV promoter43,44, GCaMP6f26 (Addgene, Plasmid #40755), and the Ps rbcS 3 A terminator45.



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